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KMID : 1094720130180050950
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Biotechnology and Bioprocess Engineering
2013 Volume.18 No. 5 p.950 ~ p.955
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Thermodynamics and kinetics of lysozyme adsorption onto two kinds of weak cation exchangers
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Cheng Yong-Mei
Jin Xiong-Hua
Gao Dong
Xia Hai-Feng
Chen Jing-Hua
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Abstract
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The present work investigated the adsorption behaviors of lysozyme onto weak cation exchangers at different temperatures. The adsorption isotherm, adsorption thermodynamics and adsorption kinetics were studied. The results indicate that the adsorption of lysozyme onto acrylic acid copolymer based beads (Hydrolite D115) is spontaneous and exothermic, while that onto agarose based beads (CM Sepharose 6 Fast Flow) is also spontaneous, but endothermic. The pseudo second-order kinetic model fits well to the dynamic adsorption experimental data, and the kinetic results are also in concert with the adsorption thermodynamics.
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KEYWORD
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ion exchange, lysozyme, agarose beads, acrylic acid based resin, thermodynamics and kinetics
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